desalting DNA

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Thu Jun 3 11:14:23 EST 1999


At 09:55 AM 6/2/99 -0700, Guy Hoelzer wrote:
>In article <199906011610.KAA23666 at nestor.NMSU.Edu>, hroychow at NMSU.EDU
>("Hiranya S. Roychowdhury") wrote:
>
>> So, if somebody wants DNA with absolutely no salt , s/he may soak the DNA
>> pellet in 70% EtOH for extended periods and do more than just one wash. This
>> is what I do when I want to get rid of salts or pigments: After the DNA is
>> pelleted/spooled out from the precipitaion, I let the pellet/spooled DNA sit
>> in 70%EtOH for 15 min before spinning/spooling out the DNA. I repeat this
>> two/three times. 
>
>Will this method get rid of excess amounts of EDTA, as well as salts like NaCl?
>
>-- 
>Guy Hoelzer
>Department of Biology
>University of Nevada Reno
>Reno, NV  89557
>
>

Yes


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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