2-mercaptoethanol and DTT

Robert Hartley rh at mblab.gla.ac.uk
Thu Jun 3 11:14:38 EST 1999

In article <PZ$7XDAGzpV3Ew9w at genesys.demon.co.uk>, "Dr. Duncan Clark"
<Duncan at genesys.demon.co.uk> wrote:

> In article <37567F17.6785C194 at yahoo.com>, Mismatch 420
> <mismtch420 at yahoo.com> writes
> >Anyone care to comment on the interchangeability(sp?) of these 2
> >compounds?
> No real problem. 10mM B-mercaptoethanol for 1mM DTT. I believe that B-
> mercaptoethanol goes off faster but is a lot cheaper than DTT for say
> medium scale protein purification i.e. buffer on 20 litre scale etc.
> Duncan 
when useing DTT make sure the pH is 8.0 or above. Apparently cleland found
that the efficacy was 10x at pH 8 cf pH7. Please check this before useing
as it is done from memory.

PS Duncan are you on CSA.*

Bob Acorn RiscPC Running RiscOS 3.6 @luss.demon.co.uk

Robert Hartley BSc(Hons).                                    
Centre for Cell Engineering,                                                       
University of Glasgow,                                  
G12 8QQ                                               
Web: http://www.gla.ac.uk/Inter/CellEngineering          
E-Mail rh at mblab.gla.ac.uk                             

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