2-mercaptoethanol and DTT
rh at mblab.gla.ac.uk
Thu Jun 3 11:14:38 EST 1999
In article <PZ$7XDAGzpV3Ew9w at genesys.demon.co.uk>, "Dr. Duncan Clark"
<Duncan at genesys.demon.co.uk> wrote:
> In article <37567F17.6785C194 at yahoo.com>, Mismatch 420
> <mismtch420 at yahoo.com> writes
> >Anyone care to comment on the interchangeability(sp?) of these 2
> No real problem. 10mM B-mercaptoethanol for 1mM DTT. I believe that B-
> mercaptoethanol goes off faster but is a lot cheaper than DTT for say
> medium scale protein purification i.e. buffer on 20 litre scale etc.
when useing DTT make sure the pH is 8.0 or above. Apparently cleland found
that the efficacy was 10x at pH 8 cf pH7. Please check this before useing
as it is done from memory.
PS Duncan are you on CSA.*
Bob Acorn RiscPC Running RiscOS 3.6 @luss.demon.co.uk
Robert Hartley BSc(Hons).
Centre for Cell Engineering,
University of Glasgow,
E-Mail rh at mblab.gla.ac.uk
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