PCR Troubleshooting References

[stewartl at alkami.com]

This month in Alkami Concise PCR Reviews, Dr. Laura Ruth continues her
discussion of PCR enhancers and inhibitors. Her article covers technical
details and includes references to the literature and related enhancer &
inhibitor links.
 
You can find her article at: http://www.alkami.com/reviews/rvwjun99.htm
 
A listing with links to the full text of previous Concise PCR Review
articles can be found at: http://www.alkami.com/reviews/indxrvws.htm

In addition, a collected list of PCR troubleshooting tips can be found
at http://www.alkami.com/methods/reftrbl.htm which includes information
on:

   Possible Components and Conditions affecting ...
      Specificity: Non-Specific Products 
      Efficiency: Little or No Product Yield 
      Multiple Product Yields or High-Molecular-Weight Smear is Observed 
      Primer-Dimers 
      Product is the Wrong Size 

   About ... 
      Inhibitors 
      Enhancers 

   How To ... 
      Reduce Contamination 

The Alkami Biosystems site, http://www.alkami.com, is devoted to
offering you a growing collection polymerase chain reaction resources.
We have online references that include primer design tips and tools,
reagent concentrations, polymerase characteristics, PCR methods, and
temperature calculations. In addition, you will find regular reviews of
PCR issues, employment resources for the biologist, as well as special
FREE lab supply offers.
 
Thanks for your interest.
---
ALKAMI BIOSYSTEMS, Inc.
Simplified Solutions for Complex Questions (tm)
info at alkami.com      http://www.alkami.com
P.O. Box 11216        Berkeley CA 94712 USA