how to isolate a large plasmid

Neil McKenna nmckenna at
Mon Jun 7 08:50:49 EST 1999

root at wrote:
> who has experience in isolating a large plasmid? I try to isolate a
> plasmid pBluescript SK(+) containing a about 18kb insert but fail.

We regularly isolate Gram negative plasmids of 20+ kb using a "large plasmid
extraction" protocol. If you want it, let me know.

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