ssDNA and dsDNA after asymetric PCR

juamber at my-deja.com juamber at my-deja.com
Tue Jun 8 02:37:48 EST 1999


Hi all,
after performing an asymetric PCR with specific primers for a certain
sequence I assume we end with the amplified fragment in the two forms of
ssDNA and dsDNA and that both are detectable on a gel(always depending
on the ratio between the two primers)?, which is which? Does ssDNA
migrate further than dsDNA?
Thanks,
Juamber.


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