mRNA isolation directly from tissue or cultured cells

Robert Hartley rh at mblab.gla.ac.uk
Tue Jun 8 03:21:45 EST 1999


In article <375be687.28278197 at news.soton.ac.uk>, kap2 at soton.ac.uk (Dr.
Kris Partridge) wrote:

> On Sun, 06 Jun 1999 15:24:16 +0200, "O. de Leeuw"
> <monique_olav.deleeuw at wxs.nl> wrotz:
> Skip the commercial kit. In our hands the method of Sacchi and
> Chomzynski (don't have reference) works the best.  It's basically a
> phenol/chloroform/guanidine extraction.  Let me know if you want to
> try this and I'll look up the exact reference.

Isn't this total RNA isolation?

cheers
Bob

> >Dear researchers,
> >
> >Who have good experience with isolations of  mRNA directly from tissue
> >or cultured cells. Which commercial kit should I try?
> >
> >Gr.
> >Olav.

-- 
Robert Hartley BSc(Hons).                                    
Centre for Cell Engineering,                                                       
University of Glasgow,                                  
Glasgow.                                               
G12 8QQ                                               
Web: http://www.gla.ac.uk/Inter/CellEngineering          
E-Mail rh at mblab.gla.ac.uk                             



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