mRNA isolation directly from tissue or cultured cells
rh at mblab.gla.ac.uk
Tue Jun 8 03:21:45 EST 1999
In article <375be687.28278197 at news.soton.ac.uk>, kap2 at soton.ac.uk (Dr.
Kris Partridge) wrote:
> On Sun, 06 Jun 1999 15:24:16 +0200, "O. de Leeuw"
> <monique_olav.deleeuw at wxs.nl> wrotz:
> Skip the commercial kit. In our hands the method of Sacchi and
> Chomzynski (don't have reference) works the best. It's basically a
> phenol/chloroform/guanidine extraction. Let me know if you want to
> try this and I'll look up the exact reference.
Isn't this total RNA isolation?
> >Dear researchers,
> >Who have good experience with isolations of mRNA directly from tissue
> >or cultured cells. Which commercial kit should I try?
Robert Hartley BSc(Hons).
Centre for Cell Engineering,
University of Glasgow,
E-Mail rh at mblab.gla.ac.uk
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