Stable Transfection of T Cells

Alan J Watson watsonaj at bms.com
Tue Jun 8 07:42:36 EST 1999


Greetings.

I need to prepare some stably transfected T cells (three
different CD4+ lines, if that matters), but I have never
transfected this type of cell before.  Any tips on lipids or
other reagents that work well on T cells in general, or do I have
determine the optimal reagent for each cell line experimentally?

Thanks in advance,

John Watson
Bristol-Myers Squibb Lead Discovery Molecular Biology
watsonaj at bms.com




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