Does Ethidium bromide retard RNA migration?

Jonathan Howarth jrh2 at
Tue Jun 8 09:08:45 EST 1999

On Mon, 7 Jun 1999, Mark Garry Petten wrote:

> I've loaded ethidium bromide with my samples and ended up with strange
> results. All the samples loaded including my ladder appear to be near the
> wells and did not migrate. Does ethidium bromide retard the migration of
> RNA samples through a gel?
> Thanks
> Mark G. Petten
> Dept. of Biology
> Memorial University of Newfoundland

Certainly not to that extent. I use this method routinely. You may have
had buffering problems or agarose concentration problems though. 

Jonathan Howarth

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