10X LB?

Shiao Wang Shiao.Wang at usm.edu
Tue Jun 8 10:17:02 EST 1999

We use LB and NZY plates for routine plating of E. coli. I've not seen
this discussed but is there a reason one can not make aliquots (50 ml)
of 10X un-autoclaved LB or NZY and store them frozen? This would
simplify the process of making LB/NZY agar for pouring small batches of
plates. Thanks for your attention and any input.

Shiao Y. Wang
Department of Biological Sciences
University of Southern Mississippi

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