'Cracking'

[Dr. Robert Gay]

Dear all

We're looking for a protocol for the rapid screening of plasmid DNA
directly from transformed E.coli colonies (avoiding the overnight liquid
culture step).

It's a protocol you may know as 'Cracking'.  We had such a protocol, once
upon a time, but have  lost it !

Hope someone can help.

Thanks in advance

Robert






Dr Robert Gay

Post Doctoral Research Fellow
CRC for Biopharmaceutical Research
Department of Biotechnology
University of New South Wales
Sydney, NSW 2052, Australia

Tel : +61(0)2-9385-1892
Fax : +61(0)2-9313-6710