Anyone tried the pIZ/V5-His vector for expression in insect cells?

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Wed Jun 9 05:53:51 EST 1999


Hi all,
Has anyone out there tried the pIZ/V5-His vector from Invitrogen for
protein expression in insect cells? It sounds almost too good to be
true: high level expression without the baculovirus work. Any
experiences? What´s the expression level one can achieve in real life?

Thanks for all input ,
Frank




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