PCR and Genomic DNA contamination

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Thu Jun 10 06:07:36 EST 1999

In article <7jmtkv$287$1 at nnrp1.deja.com>, Dandog <ddam at uh.edu> writes
>(Primers are specific for murine prepro alpha I type I and type III

Try a search of your primers against E.coli. There is always a low level
of E.coli DNA, albeit sheared, in Taq polymerase preps. It is a DNA
modifying enzyme and holds on tight! If your primers have homology to
something in E.coli and are close enough together, that may be the cause
of your problem.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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