poly A mRNA in bacteria?

"", David Alexander dalex at NEXUS.MICROIMM.MCGILL.CA
Thu Jun 10 10:37:23 EST 1999

Hello All,

I am curious to know if there are examples of bacteria that poly 
adenylate their mRNA. 
I've heard that mycobacteria do this - any others?

Without handy polyA tails, separation of mRNA, and generation of cDNA
is difficult. Is there a method/enzyme that will reliably add polyA 
to bacterial RNA?

Thanks for any insight,


dalex at microimm.mcgill.ca

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