ips98 at HOTMAIL.COM
Thu Jun 10 11:13:24 EST 1999
I am encountering a problem while trying to isolate an AFLP band of size
~250bp from the AFLP ladder i generate in a 12% acrylamide gel. The problem
is when i excise the band, perform a re-pcr and load it again to check, i
see, alongwith my desired band, a couple of bands greater and smaller than
it, that have presumably got amplified. This happens even when i cut a
tiny area of the desired band to eliminate contaminating bands.
Please tell how to get rid of the undesired bands so that i can clone the
band of my interest.
Thanks in advance,
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