AFLP problems

[inderpal singh]

Dear Netizens,
    I am encountering a problem while trying to isolate an AFLP band of size 
~250bp from the AFLP ladder i generate in a 12% acrylamide gel. The problem 
is when i excise the band, perform a re-pcr and load it again to check, i 
see, alongwith my desired band, a couple of bands greater and smaller than 
it, that have presumably got amplified.     This happens even when i cut a 
tiny area of the desired band to eliminate contaminating bands.
   Please tell how to get rid of the undesired bands so that i can clone the 
band of my interest.
Thanks in advance,
IP


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