poly A mRNA in bacteria?
Austin So (Hae Jin)
haejin at netinfo.ubc.caX
Thu Jun 10 12:21:10 EST 1999
David Alexander" wrote:
> I am curious to know if there are examples of bacteria that poly
> adenylate their mRNA.
> I've heard that mycobacteria do this - any others?
Most bacteria do produce some level (maybe "almost none" is maybe more
appropriate) of polyA mRNA, though the length of the polyA tail (10-100?
I don't know why that number comes to mind) is very small compared to
eukaryotes (200-400). This has only recently been determined (like within
the last 2 years).
> Without handy polyA tails, separation of mRNA, and generation of cDNA
> is difficult. Is there a method/enzyme that will reliably add polyA
> to bacterial RNA?
Amara and Vijaya (1997) Specific polyadenylation and purification of
total messenger RNA from Escherichia coli. Nucl. Acids Res. 25(17),
Austin P. So (Hae Jin)
University of British Columbia
E-mail: haejin at netinfo.ubc.ca
http://www.interchange.ubc.ca/haejin/index.html (under construction)
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