toxic protein

junz at sfu.ca junz at sfu.ca
Thu Jun 10 19:57:48 EST 1999


Hi there,

I have a viral capsid protein gene cloned in pET vector in BL21(DE3)pLysS cells. After 3 hours induction, I compared the lysates before and after induction on PAGE. No difference at all. What's wrong? Is the protein toxic to the host? If it is, how to eliminate? 
Anyway, the final question is how to make the protein express?

Any suggestion are highly appreciated.

Mark





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