mRNA isolation directly from tissue or cultured cells

Alexander Brands alexander.brands at uni-tuebingen.de
Wed Jun 9 06:19:32 EST 1999


> > Skip the commercial kit. In our hands the method of Sacchi and
> > Chomzynski (don't have reference) works the best.  It's basically a
> > phenol/chloroform/guanidine extraction.  Let me know if you want to
> > try this and I'll look up the exact reference.
>
> Isn't this total RNA isolation?

Well that´s true, but doing a LiCl precipitation without EtOH removes small
RNA quite effectively. Check Ambions web site for Tech Bulletin No.160.

Alexander





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