Stable mRNA's?

Alexander Brands alexander.brands at uni-tuebingen.de
Wed Jun 9 06:15:05 EST 1999


> Sorry for not mentioning!
> Caco2-cells (colon carcinoma) treated with certain lipids.

Good to know...We work with Caco2 as well and found in western blots that Villin
is a useful internal standard. Should work for mRNA, too.

Alexander


>
> Jorgen
>
> In article <Jarmo.Schrader-0706991856230001 at d177.genfys.slu.se>,
> Jarmo.Schrader at removethis.genfys.slu.se (Jarmo Schrader) wrote:
>
> > What cells? E.coli? Arabidopsis? Human? Drosophila?? Which tissue?
> >
> >
> > In article <jorgen.borrebak-0706991429450001 at pkmac45.uio.no>,
> > jorgen.borrebak at basalmed.uio.no (Jørgen Borrebæk) wrote:
> >
> > > Hi!
> > >
> > > I'm doing expression studies and need to check the stability of my
> > > reference mRNA's when cells are subjected to treatment. Does anyone know
> > > any mRNA's that's known to be stably transcribed under different
> > > influences to the cells?
> > > (like G3PDH, beta-actin or other usual kontrols)
> > >
> > > Jorgen




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