Problems with SacI

Martin Offterdinger a8803349.nospam at unet.univie.ac.at
Fri Jun 11 04:56:56 EST 1999


Hi!
I am currently facing serious problems using the  SacI restriction
enzyme. I can cut a purified plasmid with it, but I am not able to do
a double digest within the same buffer. Nor can I do the digests
sequentially, i.e. digest with second enzyme first, gel purify
fragment, redigest with Sac I. So what else could I do? I have heard
that Sac I is extremely sensitive to salt, so should I try to wash the
fragment more extensively ??
Martin
Martin Offterdinger
Internal Med.I,Dept. Oncology
University of Vienna
Austria
E-Mail:a8803349.nospam at unet.univie.ac.at
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