Problems with SacI

[Dr. Duncan Clark]

In article <3760dc7a.6592263 at news.univie.ac.at>, Martin Offterdinger
<a8803349.nospam at unet.univie.ac.at> writes
>I am currently facing serious problems using the  SacI restriction
>enzyme. 

There are a couple of inexpensive blunt-ended iso.'s of Sac I, EcoICR I
(Promega)  and Ecl136 II (I think Fermentas). They may be more
accommodating.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk