AFLP problems

Liz Parks liz_parks at ncsu.edu
Fri Jun 11 09:36:43 EST 1999


I have seen the same thing happen with AFLP bands of all different
sizes.  However, when I load this reamplified product on an agarose or
mini NON-denaturing acrylamide gel, I only see a single band of the
correct size.  Go ahead and clone your product.  When you sequence it,
you will most likely get a fragment of the correct size.  I did.  I
don't know what causes the extra banding on the denaturing PAG .  Could
be denaturation of secondary structure?

Good luck!

Liz

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Liz Parks
North Carolina State University
Department of Plant Pathology
Box 7616
Raleigh, NC  27695

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