mRNA isolation directly from tissue or cultured cells

[Carrie]

>Who have good experience with isolations of  mRNA directly from tissue
>or cultured cells. Which commercial kit should I try?

I have successfully used the QuickPrep mRNA Purification Kit from
Amersham Pharmacia Biotech for mRNA purification direct from
cultured adherent cells and subsequently used the mRNA for
successful RT-PCRs.

Carrie