mRNA isolation directly from tissue or cultured cells

Carrie Carrie at pacwalk.nospam.demon.co.uk
Fri Jun 11 20:07:38 EST 1999


>Who have good experience with isolations of  mRNA directly from tissue
>or cultured cells. Which commercial kit should I try?

I have successfully used the QuickPrep mRNA Purification Kit from
Amersham Pharmacia Biotech for mRNA purification direct from
cultured adherent cells and subsequently used the mRNA for
successful RT-PCRs.

Carrie



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