Glycerol PAGE "toughening"

[Daniel Kim]

Dear Group:

Years ago, I heard incidentally that adding glycerol to SDS-PAGE mix will 
result in a gel that is physically more durable.  I cannot remember the details, 
however.

Can anyone tell me whether this is true?  What concentration of glycerol 
is necessary?  What effect will this have on the running/blotting properties 
of the gel?

Thanks

Daniel Kim
dkim at nmsu.edu