request: mouse mAB against mouse tissue??

Frank Dehner dehner at PAN.ZOO.UNI-JENA.DE
Sun Jun 13 11:05:33 EST 1999

Hi everybody,

how can I detect an antigen in mouse nervous tissue with a primary antibody
raised in mouse (against rat protein)?
I have ascites and supernant from cell culture and no idea about the Ig
class of this AB.
How can one avoid unspecific binding of the secondary antibody (which must
be ....anti mouse) to the tissue?
Any ideas about double labelling with a second mAB?
Thanx a lot.

              Frank Dehner
           Universitaet Jena
    Institut fuer Allgemeine Zoologie
    und Tierphysiologie
              Erbertstr. 1
          07743 Jena, GERMANY

phone: +49 (0)3641-949118 (or 949100)
  fax: +49 (0)3641-949102
email: dehner at

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