Cell lysis buffer/reagents

Russell, Rachael Rachael.Russell at esr.cri.nz
Sun Jun 13 16:04:44 EST 1999


Hi there
I am looking for a good lysis reagent/buffer for lysing mammalian cells, so
that I am able to assay a cytoplasmic protein. Am currently using Trition
X-100/PBS/Water/Potassium Phosphate/Freeze Thaw cycle variations, but want a
more reliable method that doesn't degrade the protein.
The cells are adhered to a 96-well plate, and I am assaying Luciferase
activity.

Thanks
Rachael Russell
Env. Toxicology
Institute of Environmental Science and Research (ESR)
New Zealand
rachael.russell at esr.cri.nz



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