Protocol for GFP compatible BrdU staining?

Christian Zuppinger zuppinge at cell.biol.ethz.ch
Mon Jun 14 10:04:30 EST 1999


Does anybody have a GFP compatible BrdU staining protocol?
The BrdU kit, available from Roche diagnostics (Boehringer Mannheim),
uses an ethanol-glycin buffer (pH 2)
which destroys the fluoreszence of GFP. Does anybody have an alternative
protocol?
 

--
Josef P. Magyar, Ph.D.
ETH Hoenggerberg
Institut fuer Zellbiologie, HPM F27
CH-8093 Zurich
Switzerland

Tel: + 441 1 633 33 54
Fax: + 441 1 633 10 69
Email: magyar at cell.biol.ethz.ch
 
 




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