Fluorescent probe labelled dTTP

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Jun 14 09:40:59 EST 1999


In article <3764EB79.799A at cf.ac.uk>, Dr Antony Halsall
<halsalla at cf.ac.uk> writes
>Or
>alternatively is their a polymerase that contains extendase activity and
>uses ddTTP as efficiently as dTTP?

Thermosequenase or AmpliTaq FS i.e 5-3 exo minus Taq pol. with ddNTP
mutation (increases incorporation off ddNTPs about 1000 fold over wild
type).  

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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