screening for DNA-binding proteins

Andy Cannons acannons at
Mon Jun 14 12:34:33 EST 1999

We are screening a gt11 expression library for a DNA binding protein.
Just wonder if anyone has any tips out there. We are using a repeat
sequence of binding site. Background was a problem but that seems to be
eliminated. We tried denaturation and renaturation of the filters but
that lost the signal. Any suggestions to improve the signal?

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