GFP nd BrdU detection incompatibilies

Christian Zuppinger zuppinge at cell.biol.ethz.ch
Tue Jun 15 00:41:00 EST 1999


Does anybody have a GFP compatible BrdU staining protocol?
The BrdU kit, available from Roche diagnostics (Boehringer Mannheim),
uses an ethanol-glycin buffer (pH 2)
which destroys the fluoreszence of GFP. Does anybody have an alternative

protocol?
 

Josef

PS. Standard fixation with PFA and permeabilisation instead of this
ethanol-glycin buffer  does not work.
--
Josef P. Magyar, Ph.D.
ETH Hoenggerberg
Institut fuer Zellbiologie, HPM F27
CH-8093 Zurich
Switzerland

Tel: + 441 1 633 33 54
Fax: + 441 1 633 10 69
Email: magyar at cell.biol.ethz.ch
 
 
 

--

Christian Zuppinger
ETH Hoenggerberg
Institut fuer Zellbiologie, HPM F27
CH-8093 Zurich
Switzerland

Tel: + 441 1 633 33 54
Fax: + 441 1 633 10 69
Email: zuppinge at cell.biol.ethz.ch
or czuppinger at access.ch
WWW:
Institute http://www.cell.biol.ethz.ch/
 




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