QIAGEN native lysis protocol

Kevin Urbanic kurbanic at uoguelph.ca
Tue Jun 15 09:34:29 EST 1999


I am currently trying to natively purify a His-tagged protein using 
QIAGEN Ni2+ resin.  There protocol for native cell lysis is not very 
efficient.  Does anybody have any real good methods for natively lysing 
E.coli cells?


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