Quantitative PCR fine tuning

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Jun 16 03:22:08 EST 1999


In article <376701E1.A87520E2 at UNSW.edu.au>, Robert Passey
<R.Passey at UNSW.edu.au> writes
>Does anyone have any ideas on how I can either slow down the
>contaminating product or speed up the other?

Other than primer redesign, try varying the Mg concn. and/or annealing
temp. HotStart?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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