GFP and quantitation

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Thu Jun 17 02:27:03 EST 1999


Hi Stewart,


> Has anyone used GFP as a quantitative measure of expresssion in transfected
> cells?  The other questions are how?

by a fluorimetric assay. A protocol can be found, eg. in the "Living Colors"
User manual from clontech (no affil.). You can download it from their web site
www.clontech.com, protocol number PT2040-1. I haven´t tried it myself yet.

> and why?

What could be the reason to quantitate something? To know how much there is, I
guess....

Frank




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