Best substrate for Western-HRP

Martin Offterdinger a8803349.nospam at unet.univie.ac.at
Thu Jun 17 03:58:10 EST 1999


Hi!
Which substrate is currently the best for chemilum.  Western-HRP
detection. I am currently using ECL (Dupont), but I am not very
satisfied, because it has a short expiry period and sometimes gave
only very fade bands (everything else was OK.).  I am further using
PVDF membranes and I do not want to switch to Nitro. So what are the
best  most often used alternatives. Any recommendations welcome!!!
Martin
Martin Offterdinger
Internal Med.I,Dept. Oncology
University of Vienna
Austria
E-Mail:a8803349.nospam at unet.univie.ac.at
(remove .nospam before mailing)



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