SDS-PAGE and guanidine....

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Jun 17 04:44:16 EST 1999


jqpublic at home.net wrote:
> Anyone know a good way to concentrate a protein sample so that it can be
> used is SDS-PAGE if the sample is in 7M guanidine? I have sephacryl
> column fractions in said buffer but need to run 50+ uL in the gel to see
> the bands.

Can't you use urea instead of guanidine? Guanidine is ionic and therefore
causes problems with electrophoresis.

Other than that: no suggestions (to manipulate a large number of samples,
that is).

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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