SDS-PAGE and guanidine....
krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Jun 17 04:44:16 EST 1999
jqpublic at home.net wrote:
> Anyone know a good way to concentrate a protein sample so that it can be
> used is SDS-PAGE if the sample is in 7M guanidine? I have sephacryl
> column fractions in said buffer but need to run 50+ uL in the gel to see
> the bands.
Can't you use urea instead of guanidine? Guanidine is ionic and therefore
causes problems with electrophoresis.
Other than that: no suggestions (to manipulate a large number of samples,
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */
More information about the Methods