DNA quantification

[Bill_A_Nussbaumer at ms.bd.com]

Bill A Nussbaumer at BDX
06/17/99 03:48 PM


You can use oligreen, picogreen and ribogreen to quantify ssDNA, dsDNA, and RNA.
You can get them in the form of a kit complete with nucleic acid standards.
You'll find them from Molecular Probes at http://www.probes.com.  They are
fluorescent dyes when bound to polynucleic acids.  Just do a search on their
website and you'll be able to view the package insert (i.e. procedure) using
adobe acrobat.  No affiliation, I just use the stuff .... (so the net police
don't jump on my back.)

Bill





I would like to know if anybody has a method of determining [DNA] other
than using a UV-vis spectrophotometer.  Our departmental spec seems to
provided extremely inconsistent concentrations when I spec plasmid DNA
that is at a concentration less than 1ug/ml.


Kevin Urbanic