request: mouse mAB against mouse tissue??

[Scott Craig]

Dear Frank,
You could try labelling your primary mouse antibody with biotin and then using
a avidin/streptavidin conjugate as the secondary. This shouldn't have any cross
reactivity with other mouse antibodies present.

Frank Dehner wrote:

> Hi everybody,
>
> how can I detect an antigen in mouse nervous tissue with a primary antibody
> raised in mouse (against rat protein)?
> I have ascites and supernant from cell culture and no idea about the Ig
> class of this AB.
> How can one avoid unspecific binding of the secondary antibody (which must
> be ....anti mouse) to the tissue?
> Any ideas about double labelling with a second mAB?
> Thanx a lot.
> Frank
>
> ::::::::::::::::::::::::::::::::::::::
>               Frank Dehner
>            Universitaet Jena
>   Biologisch-Pharmazeutische-Fakultaet
>     Institut fuer Allgemeine Zoologie
>     und Tierphysiologie
>               Erbertstr. 1
>           07743 Jena, GERMANY
>
> phone: +49 (0)3641-949118 (or 949100)
>   fax: +49 (0)3641-949102
> email: dehner at pan.zoo.uni-jena.de
> ::::::::::::::::::::::::::::::::::::::

--
Scott Craig
Dept. of Biotechnology
University of New South Wales
Sydney, Australia