[Q] 18% SDS-PAGE

Namjin Chung n.chung at duke.edu
Thu Jun 17 20:30:41 EST 1999


I'm trying to work on 18% tris-glycine SDS-PAGE for 7-9kDa proteins.
Somehow, when I run the gel, dye smears broadly behind buffer front.
Also, western blot shows smeared bands.  Why does this happens?


Namjin Chung
Department of Pharmacology & Cancer Biology
Department of Genetics
Howard Hughes Medical Institute
Duke University Medical Center





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