G418/Geneticin

[Dima Klenchin]

In article <ejdunphy-1606991124510001 at peds164.medsch.wisc.edu>, ejdunphy at facstaff.wisc.edu (Edward Dunphy) wrote:
:I was wondering if anyone knew the usual concentration of G418 to use for
:selection of stable transfected tumor cell lines.
:

Depends on cell line. Anywhere between 0.3 and 1.0 mg/ml and varies
slightly from vendor to vendor and from batch to batch (the powder 
is not pure G418). You better determine optimum yourself. 

        - Dima