protein MW

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Fri Jun 18 09:45:39 EST 1999


In article <7jqt4t$dj1$1 at nnrp1.deja.com>, Kresten <kresten at my-deja.com> wrote:
:
:> I would like to know the MW of a 788aa protein. Could anyone tell me
:> how to calculate the MW if I know the sequence?
:
:Just go to:
:http://www.expasy.ch/tools/protparam.html
:and paste your sequence
:
:This will give you MW and estimations of other parameters.

That's if you know the sequence (just add up all aa in the 
protein). Average aa MW is 100. For most practical reasons your protein's
MW is 80 kDa. Estimates from SDS PAGE or gel-filtration are no better
than that. 

        - Dima






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