[Q] 18% SDS-PAGE

L.D. lnd at mail.utexas.edu
Fri Jun 18 15:02:09 EST 1999


Andreas Weber wrote:

> Namjin Chung wrote:
> >
> > I'm trying to work on 18% tris-glycine SDS-PAGE for 7-9kDa proteins.
> > Somehow, when I run the gel, dye smears broadly behind buffer front.
> > Also, western blot shows smeared bands.  Why does this happens?
> >
> > Namjin Chung
> > Department of Pharmacology & Cancer Biology
> > Department of Genetics
> > Howard Hughes Medical Institute
> > Duke University Medical Center
>
> So why don't you try tris/tricine-gels according to Schaegger and Jagow
> (1987), Anal Biochem 166:368-379.
> This system works perfectely for separation of proteins with an apparent
> mw in the range of 100 - 2 kDa.
>
> Andreas
>

Tris-tricine system can solve your problem almost completely. And, that
reference is a certain one for that. You can increase slightly Bis.Acr.
concentration and add some glycerol to gel buffer.

L.D.




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