[Q] 18% SDS-PAGE

Peter pxpst2 at vms.cis.pitt.edu
Sat Jun 19 10:38:37 EST 1999


15 % tris tricine gel will reslove fragments of this size quite easily. 

In article <376A7E2F.6E2346FD at pitt.edu>, rdudley+ at pitt.edu wrote:

> > I'm trying to work on 18% tris-glycine SDS-PAGE for 7-9kDa proteins.
> > Somehow, when I run the gel, dye smears broadly behind buffer front.
> > Also, western blot shows smeared bands.  Why does this happens?
> >
> > Namjin Chung
> > Department of Pharmacology & Cancer Biology
> > Department of Genetics
> > Howard Hughes Medical Institute
> > Duke University Medical Center
> 
> Another thing to try is using Tris-tricine buffer and Amresco's
> PAGE-Plus acrylamide.  I've used it for proteins that small at the 14%
> or 16% range.  Just make the gel using the standard recipe (adjusted for
> % of the stock sol'n), and substitute the PAGE-Plus for the acrylamide.
> 
> The traditional methods don't resolve proteins that small very well.  At
> that range, the pore size of the polyacrylamide doesn't sieve well, and
> your proteins are jumbled around with the SDS micelles.



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