QIAGEN native lysis protocol

Peter a9001527 at unet.univie.ac.at
Tue Jun 22 00:48:43 EST 1999

On 15 Jun 1999 14:34:29 GMT, kurbanic at uoguelph.ca (Kevin Urbanic)

>I am currently trying to natively purify a His-tagged protein using 
>QIAGEN Ni2+ resin.  There protocol for native cell lysis is not very 
>efficient.  Does anybody have any real good methods for natively lysing 
>E.coli cells?

Maybe the B-PER reagent from Pierce is the thing you are looking for.

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