Protocol for GFP compatible BrdU staining?

Nick ihanvan at imvs.sa.gov.au
Mon Jun 21 22:54:02 EST 1999


Try Holm et al. in Cytometry 32:28-36 (1998)

It describes a method for using BrdU with paraformaldehyde/NP40 for
simultaneous analysis of: BrdU uptake, cell surface markers and ploidy.

May be what you are after.

Nick van Holst
Research Assistant

Christian Zuppinger wrote in message
<376519FB.F315D2AB at cell.biol.ethz.ch>...
>Does anybody have a GFP compatible BrdU staining protocol?
>The BrdU kit, available from Roche diagnostics (Boehringer Mannheim),
>uses an ethanol-glycin buffer (pH 2)
>which destroys the fluoreszence of GFP. Does anybody have an alternative
>protocol?
>
>
>--
>Josef P. Magyar, Ph.D.
>ETH Hoenggerberg
>Institut fuer Zellbiologie, HPM F27
>CH-8093 Zurich
>Switzerland
>
>Tel: + 441 1 633 33 54
>Fax: + 441 1 633 10 69
>Email: magyar at cell.biol.ethz.ch
>
>
>





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