Bacterial conjugation

[Andrup]

Paul Beare wrote:
> 
> Does anyone have a reference for conjugation methods. I'm currently having
> problems getting transconjugants using the E.coli strain S-17 (donor) and
> the Pseudomonas aeruginosa strain PAo1 under selection for both
> tetracycline and kanamycin.
> 
> Paul Beare  B.S.c (Hons)
> 

Please tell us more about the conjugation system you are using. Some
conjugative plasmids require solid surfaces for effecient conjugation.
The frequencies are dependent on the nature of recipient (RM-systems,
receptors etc.), the conjugationsystem(the plasmid) and various physical
(shaking, D:R-ratio, temp), biological (growth phase - most systems have
optimal effeciencies during exponential growth), and chemical (media,
lack of inhibitors) conditions.

Are you mobilizing a non-conjugative plasmid by a conjugative plasmid or
is it the transfer of the conjugative plasmid you are interested in?

Good luck

yours


Lars

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*   Lars Andrup, phd  -  National Inst.Occup.Health   *
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