? on protein extraction

Ruben Zamora zamorar at pitt.edu
Tue Jun 22 10:11:00 EST 1999


Hello,

I would like to isolate protein from cultured cells to perform Western
blot. What is the best method for extraction, to lyse the cells by
adding Triton X-100 to the buffer or just leaving Triton X-100 out the
extraction buffer and completing 4 or 5 freeze-thaw cycles?

Thank you,

Ruben




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