DNA quantification

George Rutherford gruther at bilbo.bio.purdue.edu
Tue Jun 22 14:52:30 EST 1999


In article <7kb26p$2bv$1 at testinfo.uoguelph.ca>, kurbanic at uoguelph.ca
(Kevin Urbanic) wrote:

> I would like to know if anybody has a method of determining [DNA] other 
> than using a UV-vis spectrophotometer.  Our departmental spec seems to 
> provided extremely inconsistent concentrations when I spec plasmid DNA 
> that is at a concentration less than 1ug/ml.  Specifcally, I was 
> wondering if anybody has calculated the concentration of each band in a 
> 1kb DNA ladder?  I thought that could be a useful tool for "eyeing" the 
> DNA concentration.

Fluorimetry



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