Electroporation of embryos and explants

Dirk Junghans junghans at PAN.ZOO.UNI-JENA.DE
Tue Jun 22 16:02:48 EST 1999


Hi out there!

I'm planning to buy an electroporation system.
IIt is intended mainly to be used for  mammalian cell and  embryo
transfection. If possible it should work with bacteria too. Can anyone
recommend  which machine is worthfull to buy? How big is the difference
between square wave or exponential decay in embryonic transfection?And
last question has anyone experiences in transfecting brain explants with
electroporation?
Thanxs, Dirk
--
***********************************
Dirk Junghans
Inst. f. allg. Zoologie
FSU Jena
Erbertstr. 1
07743 Jena
Tel. +49(0)3641-949-118
Fax  +49(0)3641-949-102
email junghans at pan.zoo.uni-jena.de





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