imidazole and degradation

Eva Horak EM_Horak at
Tue Jun 22 16:41:40 EST 1999

We were wondering if anyone out there has experienced degradation of
their his-tagged protein by high concentrations of imidazole (250 mM for
example) when eluting from a nickel column?  Is there better way to
elute proteins from these columns to avoid degradation?
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