imidazole and degradation

[Eva Horak]

Hi!
We were wondering if anyone out there has experienced degradation of
their his-tagged protein by high concentrations of imidazole (250 mM for
example) when eluting from a nickel column?  Is there better way to
elute proteins from these columns to avoid degradation?
Thanks.
-Lily
-------------- next part --------------
A non-text attachment was scrubbed...
Name: EM_Horak.vcf
Type: text/x-vcard
Size: 142 bytes
Desc: Card for Eva Horak
Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/19990622/43c4ba8b/EM_Horak.bin