? on protein extraction

[Rich Dudley]

Ruben Zamora wrote:

> Hello,
>
> I would like to isolate protein from cultured cells to perform Western
> blot. What is the best method for extraction, to lyse the cells by
> adding Triton X-100 to the buffer or just leaving Triton X-100 out the
> extraction buffer and completing 4 or 5 freeze-thaw cycles?
>
> Thank you,
>
> Ruben

Try this:

"Total protein extraction from cultured cells for use in electrophoresis
and western blotting"  Wang, et al.  Biotechniques 20:662-668

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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