Primers for sequencing from EMBL4?

Allen Gathman agathman.nospam at biology.semo.edu
Wed Jun 23 07:46:48 EST 1999


I have an old genomic library in EMBL4, and would like to sequence some
clones from it.  Unlike most newer vectors, EMBL4 doesn't have T3, T7, or
any other standard flanking promoter to use as a sequencing primer.  Does
anyone have experience sequencing from EMBL4?  Maybe someone has already
generated some flanking primers and could send me sequences for them, so I
can save the trouble of designing and testing new ones?

--
Allen Gathman
agathman at biology.semo.edu
http://biology.semo.edu/agathman/





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